Hotspot

trnR-atpA

LSC · 10721-11244 · 524 bp

Back to Trichosanthes

Pi 0.0312

MarkerSeek score 79.10

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 57
pi: 0.0312
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	TGGAATGAAAAGCGTCCATTG	TCCACCAAGACATTCACCAA	42.9	45.0	57.4	57.0	700-754 bp	1.0	67.9
trnR-atpA_p2	TGGAATGAAAAGCGTCCATTG	TTCCACCAAGACATTCACCA	42.9	45.0	57.4	57.0	701-755 bp	1.0	67.9
trnR-atpA_p3	GGAATGAAAAGCGTCCATTGT	TCCACCAAGACATTCACCAA	42.9	45.0	57.4	57.0	699-753 bp	1.0	67.9
trnR-atpA_p4	GGAATGAAAAGCGTCCATTGT	TTCCACCAAGACATTCACCA	42.9	45.0	57.4	57.0	700-754 bp	1.0	67.9
trnR-atpA_p5	TGGAATGAAAAGCGTCCATTG	CTTCCACCAAGACATTCACCA	42.9	47.6	57.4	58.1	702-756 bp	1.0	67.1