Hotspot

trnG-trnR

LSC · 10427-10648 · 222 bp

Back to Trichosanthes

Pi 0.0229

MarkerSeek score 67.20

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 22
pi: 0.0229
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	302-380 bp	1.0	72.6
trnG-trnR_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	305-383 bp	1.0	72.3
trnG-trnR_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	308-386 bp	1.0	71.8
trnG-trnR_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	311-389 bp	1.0	71.5
trnG-trnR_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	372-450 bp	1.0	69.8