Hotspot

psbI-trnS

LSC · 8694-8800 · 107 bp

Back to Trichosanthes

Pi 0.0378

MarkerSeek score 73.10

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 107
variable_sites: 13
pi: 0.0378
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	TTCCTCTCTAATGACCCGGG	TGGACTAAAGCGTCGGATTG	55.0	50.0	58.2	58.0	216-225 bp	1.0	87.3
psbI-trnS_p2	TTCCTCTCTAATGACCCGGG	GTGGACTAAAGCGTCGGATT	55.0	50.0	58.2	58.0	217-226 bp	1.0	87.3
psbI-trnS_p3	TTCCTCTCTAATGACCCGGG	GACTAAAGCGTCGGATTGCT	55.0	50.0	58.2	58.4	214-223 bp	1.0	84.5
psbI-trnS_p4	TTCCTCTCTAATGACCCGGG	CTGAGTGGACTAAAGCGTCG	55.0	55.0	58.2	58.4	221-230 bp	1.0	84.2
psbI-trnS_p5	TTCCTCTCTAATGACCCGGG	ATTTGGGGAGAGATGGCTGA	55.0	50.0	58.2	58.4	237-246 bp	1.0	83.6