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psbK-psbI

LSC · 8208-8582 · 375 bp

Back to Trichosanthes

Pi 0.0234

MarkerSeek score 62.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 25
pi: 0.0234
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCTGCCGTAAGCTTTCGATA	TTACGTCCCGGGTCATTAGA	50.0	50.0	58.4	57.9	487-491 bp	1.0	80.0
psbK-psbI_p2	GCTGCCGTAAGCTTTCGATA	CCCGGGTCATTAGAGAGGAA	50.0	55.0	58.4	58.2	481-485 bp	1.0	79.5
psbK-psbI_p3	GCTGCCGTAAGCTTTCGATA	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.4	57.3	503-507 bp	1.0	75.7
psbK-psbI_p4	GCTGCCGTAAGCTTTCGATA	TACGTCCCGGGTCATTAGAG	50.0	55.0	58.4	58.3	487 bp	0.071429	36.9
psbK-psbI_p5	GCTGCCGTAAGCTTTCGATA	GTCCCGGGTCATTAGAGAGG	50.0	60.0	58.4	59.0	484 bp	0.071429	31.7