Hotspot

trnK-rps16

LSC · 4221-5157 · 937 bp

Back to Trichosanthes

Pi 0.0238

MarkerSeek score 68.20

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 74
pi: 0.0238
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AGTCGCGGTCTTACAAACTC	CCCAATGAGCCGTTTATCGA	50.0	50.0	57.9	58.1	1250-1315 bp	1.0	88.0
trnK-rps16_p2	GTCGCGGTCTTACAAACTCT	CCCAATGAGCCGTTTATCGA	50.0	50.0	57.9	58.1	1249-1314 bp	1.0	88.0
trnK-rps16_p3	GTCGCGGTCTTACAAACTCT	TCCCAATGAGCCGTTTATCG	50.0	50.0	57.9	58.1	1250-1315 bp	1.0	88.0
trnK-rps16_p4	ACCCGACTTGACTTGCTTTT	CCCAATGAGCCGTTTATCGA	45.0	50.0	57.9	58.1	1410 bp	0.071429	42.0
trnK-rps16_p5	ACCCGACTTGACTTGCTTTT	TCCCAATGAGCCGTTTATCG	45.0	50.0	57.9	58.1	1411 bp	0.071429	42.0