Hotspot

rps16

LSC · 4493-5589 · 1097 bp

Back to Trichoneura

Pi 0.00462

MarkerSeek score 49.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1097
variable_sites: 14
pi: 0.00462
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGGATATTAATGAACCTCCCT	CACTTTGCAATGAAATTAGAGGA	40.9	34.8	55.7	55.5	1174-1182 bp	1.0	41.0
rps16_p2	TGAACCTCCCTTACTTTTTCA	CACTTTGCAATGAAATTAGAGGA	38.1	34.8	54.7	55.5	1163-1171 bp	1.0	41.0
rps16_p3	AAGGGATATTAATGAACCTCCCT	CACTTTGCAATGAAATTAGAGGA	39.1	34.8	56.5	55.5	1175-1183 bp	1.0	41.0
rps16_p4	AGGGATATTAATGAACCTCCCTT	CACTTTGCAATGAAATTAGAGGA	39.1	34.8	56.5	55.5	1174-1182 bp	1.0	41.0
rps16_p5	AGGGATATTAATGAACCTCCCT	CACTTTGCAATGAAATTAGAGGAA	40.9	33.3	55.7	56.2	1174-1182 bp	1.0	41.0