Hotspot

rpl16

LSC · 77614-79110 · 1497 bp

Back to Trichoneura

Pi 0.0069

MarkerSeek score 57.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 28
pi: 0.0069
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTCTCCAGAAGGAAAACCCG	GGGTAGACTTCCCCTCCAAA	55.0	55.0	57.9	58.3	1769-1810 bp	1.0	80.1
rpl16_p2	CTCTCCAGAAGGAAAACCCG	GCCGAATGCATCAAAAAGGG	55.0	50.0	57.9	58.4	1786-1827 bp	1.0	79.9
rpl16_p3	TCTTTCGAGAGGCATTTGGG	GGGTAGACTTCCCCTCCAAA	50.0	55.0	57.9	58.3	1988-2029 bp	1.0	79.6
rpl16_p4	TCTTTCGAGAGGCATTTGGG	GCCGAATGCATCAAAAAGGG	50.0	50.0	57.9	58.4	2005-2046 bp	1.0	79.4
rpl16_p5	TCAGCAATAGTGTCCTTGCC	GGGTAGACTTCCCCTCCAAA	50.0	55.0	57.9	58.3	2357-2398 bp	1.0	79.1