Hotspot

ndhA

SSC · 110772-112863 · 2092 bp

Back to Trichoneura

Pi 0.00188

MarkerSeek score 59.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2092
variable_sites: 10
pi: 0.00188
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGAGAACTTTTGCGT	ATGGGGGAAGTTGATCGTTG	38.1	50.0	56.6	57.9	2165-2169 bp	1.0	60.4
ndhA_p2	TGAGAGAACTTTTGCGTTGA	ATGGGGGAAGTTGATCGTTG	40.0	50.0	55.5	57.9	2161-2165 bp	1.0	59.9
ndhA_p3	TGTTTGAGAGAACTTTTGCGT	GGGGGAAGTTGATCGTTGAA	38.1	50.0	56.6	57.8	2163-2167 bp	1.0	59.9
ndhA_p4	TGAGAGAACTTTTGCGTTGA	GGGGGAAGTTGATCGTTGAA	40.0	50.0	55.5	57.8	2159-2163 bp	1.0	59.4
ndhA_p5	TGAGAGAACTTTTGCGTTGAAG	ATGGGGGAAGTTGATCGTTG	40.9	50.0	57.5	57.9	2161-2165 bp	1.0	59.3