Hotspot

rpl32-trnL-UAG

SSC · 104790-105201 · 412 bp

Back to Trichoneura

Pi 0.0162

MarkerSeek score 70.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 412
variable_sites: 15
pi: 0.0162
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTTTTGAGCGGCAATGAGC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	486-500 bp	1.0	83.7
rpl32-trnL-UAG_p2	GCAGCCAAAACCAAAAGGTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	468-482 bp	1.0	83.6
rpl32-trnL-UAG_p3	ATTTTTGAGCGGCAATGAGC	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	479-493 bp	1.0	83.3
rpl32-trnL-UAG_p4	ATTTTTGAGCGGCAATGAGC	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.0	58.2	500-514 bp	1.0	83.1
rpl32-trnL-UAG_p5	ATTTTTGAGCGGCAATGAGC	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.0	57.8	484-498 bp	1.0	83.1