Hotspot

trnP-UGG-psaJ

LSC · 64199-64604 · 406 bp

Back to Trichoneura

Pi 0.00164

MarkerSeek score 55.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 406
variable_sites: 2
pi: 0.00164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	491-516 bp	1.0	82.6
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	492-517 bp	1.0	82.2
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	499-524 bp	1.0	82.0
trnP-UGG-psaJ_p4	ATCTCCCCGTTACAGTCCAT	AAACCTGCTAAAGCCCCAAA	50.0	45.0	57.8	57.9	571-596 bp	1.0	81.6
trnP-UGG-psaJ_p5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	496-521 bp	1.0	80.3