Hotspot

atpI-atpH

LSC · 31562-32368 · 807 bp

Back to Trichoneura

Pi 0.00315

MarkerSeek score 56.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 7
pi: 0.00315
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CGAATCCATGGAGGGTCATC	CTCTACAGCTTGTCCTGCAG	55.0	55.0	57.8	58.0	817-943 bp	1.0	83.2
atpI-atpH_p2	CGAATCCATGGAGGGTCATC	AGCCAATCCAGCAGCAATAA	55.0	45.0	57.8	57.9	754-880 bp	1.0	82.1
atpI-atpH_p3	CGAATCCATGGAGGGTCATC	GCAGTACCTTGACCAACTCC	55.0	55.0	57.8	58.2	798-924 bp	1.0	81.7
atpI-atpH_p4	CGAATCCATGGAGGGTCATC	AAGCAGCAGCAATTAGTGGA	55.0	45.0	57.8	57.8	731-857 bp	1.0	81.6
atpI-atpH_p5	CGAATCCATGGAGGGTCATC	GCCAATCCAGCAGCAATAAC	55.0	50.0	57.8	57.8	753-879 bp	1.0	81.5