Hotspot

petN-trnC-GCA

LSC · 17699-18430 · 732 bp

Back to Trichoneura

Pi 0.0051

MarkerSeek score 60.40

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 10
pi: 0.0051
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTTCTCCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	836-845 bp	1.0	73.0
petN-trnC-GCA_p2	GTCCACTTCTCCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	835-844 bp	1.0	73.0
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	777-786 bp	1.0	72.9
petN-trnC-GCA_p4	GTCCACTTCTCCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	837-846 bp	1.0	72.6
petN-trnC-GCA_p5	AGTCCACTTCTCCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	838-847 bp	1.0	72.5