Hotspot

trnG-GCC-trnG-UCC

LSC · 12583-13119 · 537 bp

Back to Trichoneura

Pi 0.00647

MarkerSeek score 53.70

Primer available yes

Label trnG-GCC-trnG-UCC

Feature Metadata

label_name: trnG-GCC-trnG-UCC
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 9
pi: 0.00647
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnG-UCC_p1	GAAGGTACGGGTTCGATTCC	AGCCTTCCAAGCTAACGATG	55.0	50.0	58.1	58.0	609-619 bp	1.0	81.4
trnG-GCC-trnG-UCC_p2	GCGAGCGTAGTTCAATGGTA	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.1	58.0	649-659 bp	1.0	81.2
trnG-GCC-trnG-UCC_p3	GAAGGTACGGGTTCGATTCC	CCTAGCCTTCCAAGCTAACG	55.0	55.0	58.1	58.1	612-622 bp	1.0	81.2
trnG-GCC-trnG-UCC_p4	GCGAGCGTAGTTCAATGGTA	CCTAGCCTTCCAAGCTAACG	50.0	55.0	58.1	58.1	652-662 bp	1.0	80.9
trnG-GCC-trnG-UCC_p5	CGTAGCGAGCGTAGTTCAAT	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.2	58.0	653-663 bp	1.0	80.4