Hotspot

rpl16-rps3

Genome · 86162-86288 · 127 bp

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Pi 0.0383

MarkerSeek score 66.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: Genome
length_bp: 127
variable_sites: 11
pi: 0.0383
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	ACCGCGAAGTGAAATTCCTT	AGGTTTGGATATTTCAAGGCA	45.0	38.1	58.1	55.4	222-228 bp	1.0	56.6
rpl16-rps3_p2	ACCGCGAAGTGAAATTCCTT	AAGGTTTGGATATTTCAAGGCA	45.0	36.4	58.1	56.2	223-229 bp	1.0	54.8
rpl16-rps3_p3	TGTTCGTTTGGGACTAAGCA	AGGTTTGGATATTTCAAGGCA	45.0	38.1	57.4	55.4	171-177 bp	1.0	54.2
rpl16-rps3_p4	ACCGCGAAGTGAAATTCCTT	AGGTTTGGATATTTCAAGGCAT	45.0	36.4	58.1	56.0	222-228 bp	1.0	53.1
rpl16-rps3_p5	ACCGCGAAGTGAAATTCCTT	AAAGGTTTGGATATTTCAAGGCA	45.0	34.8	58.1	56.9	224-230 bp	1.0	52.4