Hotspot

clpP-psbB

Genome · 76454-76857 · 404 bp

Back to Trichomanes

Pi 0.0262

MarkerSeek score 71.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 404
variable_sites: 23
pi: 0.0262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTCTTCCCCAGGTAGACGAA	ACAACAGTGTGAACGCGATA	55.0	45.0	58.2	57.9	486-487 bp	1.0	82.2
clpP-psbB_p2	CTCTTCCCCAGGTAGACGAA	TACAACAGTGTGAACGCGAT	55.0	45.0	58.2	57.9	487-488 bp	1.0	82.2
clpP-psbB_p3	CTCTTCCCCAGGTAGACGAA	AGAAACCAAAGCCGTATGCA	55.0	45.0	58.2	58.1	544-545 bp	1.0	82.1
clpP-psbB_p4	CTATACGTCGACCCAAACCG	AGAAACCAAAGCCGTATGCA	55.0	45.0	58.2	58.1	568-569 bp	1.0	81.8
clpP-psbB_p5	ATGCAAGAACAAGTCGCTCT	AGAAACCAAAGCCGTATGCA	45.0	45.0	57.8	58.1	586-587 bp	1.0	81.6