Hotspot

atpB-rbcL

Genome · 60205-60882 · 678 bp

Back to Trichomanes

Pi 0.0222

MarkerSeek score 66.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 678
variable_sites: 35
pi: 0.0222
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGCTACATCTAATACGGGGC	GCATCGACCCTTGTAACGAT	55.0	50.0	58.0	58.1	948-1027 bp	1.0	87.1
atpB-rbcL_p2	CGCTACATCTAATACGGGGC	CAGCAACGGGTTCGATATCA	55.0	50.0	58.0	58.1	970-1049 bp	1.0	87.0
atpB-rbcL_p3	CGCTACATCTAATACGGGGC	AAATCGAGTCCACCACGAAG	55.0	50.0	58.0	57.9	1292-1371 bp	1.0	86.2
atpB-rbcL_p4	CGCTACATCTAATACGGGGC	TGGAGTCATTCGGAAAGCTG	55.0	50.0	58.0	57.9	906-907 bp	0.6	64.9
atpB-rbcL_p5	CGCTACATCTAATACGGGGC	GTGGAGTCATTCGGAAAGCT	55.0	50.0	58.0	57.9	907-908 bp	0.6	64.9