Hotspot

trnF-GAA-ndhJ

Genome · 54842-55063 · 222 bp

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Pi 0.0523

MarkerSeek score 73.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 222
variable_sites: 27
pi: 0.0523
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	TCATTAGCCGGGATAGCTCA	CGAGATCCCACAGGTGAAAG	50.0	55.0	58.0	58.0	701-702 bp	1.0	83.9
trnF-GAA-ndhJ_p2	ATTAGCCGGGATAGCTCAGT	CGAGATCCCACAGGTGAAAG	50.0	55.0	58.0	58.0	699-700 bp	1.0	83.9
trnF-GAA-ndhJ_p3	AGTCATTAGCCGGGATAGCT	CGAGATCCCACAGGTGAAAG	50.0	55.0	58.0	58.0	703-704 bp	1.0	83.8
trnF-GAA-ndhJ_p4	TCATTAGCCGGGATAGCTCA	TTGGCCTTGTCTAGCTGTTG	50.0	50.0	58.0	58.1	674-675 bp	1.0	83.2
trnF-GAA-ndhJ_p5	ATTAGCCGGGATAGCTCAGT	TTGGCCTTGTCTAGCTGTTG	50.0	50.0	58.0	58.1	672-673 bp	1.0	83.2