Hotspot

trnL-CAA-trnF-GAA

Genome · 54401-54768 · 368 bp

Back to Trichomanes

Pi 0.0342

MarkerSeek score 67.20

Primer available yes

Label trnL-CAA-trnF-GAA

Feature Metadata

label_name: trnL-CAA-trnF-GAA
feature_type: igs
region: Genome
length_bp: 368
variable_sites: 27
pi: 0.0342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnF-GAA_p1	AATCCGCTGGTCTTTGTAGC	TCTACCGACTGAGCTATCCC	50.0	55.0	58.3	57.7	441-492 bp	1.0	83.4
trnL-CAA-trnF-GAA_p2	AATCCGCTGGTCTTTGTAGC	GACTTGAACTGGTGACACGA	50.0	50.0	58.3	57.8	479-530 bp	1.0	83.3
trnL-CAA-trnF-GAA_p3	AATCCGCTGGTCTTTGTAGC	ACTTGAACTGGTGACACGAG	50.0	50.0	58.3	57.8	478-529 bp	1.0	83.2
trnL-CAA-trnF-GAA_p4	AATCCGCTGGTCTTTGTAGC	AGACTTGAACTGGTGACACG	50.0	50.0	58.3	57.8	480-531 bp	1.0	83.2
trnL-CAA-trnF-GAA_p5	AATCCGCTGGTCTTTGTAGC	ATCCTCTGCTCTACCGACTG	50.0	55.0	58.3	58.3	450-501 bp	1.0	82.9