Hotspot

rps4-trnL-CAA

Genome · 53055-53829 · 775 bp

Back to Trichomanes

Pi 0.0329

MarkerSeek score 69.90

Primer available yes

Label rps4-trnL-CAA

Feature Metadata

label_name: rps4-trnL-CAA
feature_type: igs
region: Genome
length_bp: 775
variable_sites: 54
pi: 0.0329
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-CAA_p1	ACGGGGTCCTCTATAACGAG	GCGTCTACCATTTCGCCATA	55.0	50.0	58.0	58.1	823-853 bp	1.0	85.1
rps4-trnL-CAA_p2	ACGGGGTCCTCTATAACGAG	GCTCAGGATTGCCTACCAAA	55.0	50.0	58.0	57.9	921-951 bp	1.0	84.6
rps4-trnL-CAA_p3	ACGGGGTCCTCTATAACGAG	GCTTCCATTGAGTCTCTGCA	55.0	50.0	58.0	57.9	990-1020 bp	1.0	84.4
rps4-trnL-CAA_p4	TCTGTTTCGCTTCCAAACGA	GCTTCCATTGAGTCTCTGCA	45.0	50.0	58.1	57.9	1129-1159 bp	1.0	83.5
rps4-trnL-CAA_p5	AGAAGGGCTTGATCCACTCT	GCTTCCATTGAGTCTCTGCA	50.0	50.0	58.0	57.9	1407-1437 bp	1.0	83.3