Hotspot

trnE-TTC-trnG-GCC

Genome · 37320-38001 · 682 bp

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Pi 0.0306

MarkerSeek score 71.70

Primer available yes

Label trnE-TTC-trnG-GCC

Feature Metadata

label_name: trnE-TTC-trnG-GCC
feature_type: igs
region: Genome
length_bp: 682
variable_sites: 48
pi: 0.0306
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnG-GCC_p1	GTCGGCTCCTTGAAAGAGAG	TCTTTGCCAAGGAGAAGACG	55.0	50.0	58.0	57.8	772-784 bp	1.0	84.3
trnE-TTC-trnG-GCC_p2	TCGGCTCCTTGAAAGAGAGA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	58.1	57.8	771-783 bp	1.0	83.7
trnE-TTC-trnG-GCC_p3	ATGTCCTAGGCCTCTAGACG	TCTTTGCCAAGGAGAAGACG	55.0	50.0	57.7	57.8	752-764 bp	1.0	82.2
trnE-TTC-trnG-GCC_p4	CCTAGGCCTCTAGACGATGG	TCTTTGCCAAGGAGAAGACG	60.0	50.0	58.5	57.8	748-760 bp	1.0	80.8
trnE-TTC-trnG-GCC_p5	CTAGGCCTCTAGACGATGGG	TCTTTGCCAAGGAGAAGACG	60.0	50.0	58.5	57.8	747-759 bp	1.0	80.8