Hotspot

psbM-trnD-GTC

Genome · 35999-36604 · 606 bp

Back to Trichomanes

Pi 0.0432

MarkerSeek score 78.30

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: Genome
length_bp: 606
variable_sites: 58
pi: 0.0432
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	CAATTAATGCTCCGGGACCA	CTCTCTTTCAAGGAGCCGAC	50.0	55.0	57.9	58.0	1599-1734 bp	1.0	89.5
psbM-trnD-GTC_p2	AAATGAGAAAAGCTGCCGGA	CTCTCTTTCAAGGAGCCGAC	45.0	55.0	58.1	58.0	1466-1601 bp	1.0	89.2
psbM-trnD-GTC_p3	CAATTAATGCTCCGGGACCA	TTTGACAGGAGACGGACTCT	50.0	50.0	57.9	58.0	1444-1550 bp	0.8	81.5
psbM-trnD-GTC_p4	AAATGAGAAAAGCTGCCGGA	TTTGACAGGAGACGGACTCT	45.0	50.0	58.1	58.0	1311-1417 bp	0.8	81.3
psbM-trnD-GTC_p5	CAATTAATGCTCCGGGACCA	GTTTGACAGGAGACGGACTC	50.0	55.0	57.9	57.9	1445-1551 bp	0.8	81.0