Hotspot

psbA-matK

Genome · 7330-8013 · 684 bp

Back to Trichomanes

Pi 0.0355

MarkerSeek score 70.70

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: Genome
length_bp: 684
variable_sites: 55
pi: 0.0355
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	CGGAAGCTAAGTTCCCACTC	GTTGGCTCCATTGCTATCGA	55.0	50.0	58.0	58.0	1664-1676 bp	1.0	83.8
psbA-matK_p2	CGGAAGCTAAGTTCCCACTC	TCGTTGGCTCCATTGCTATC	55.0	50.0	58.0	58.0	1666-1678 bp	1.0	83.8
psbA-matK_p3	CGGAAGCTAAGTTCCCACTC	TTACGTCCTAAAATCCGCCG	55.0	50.0	58.0	58.1	1699-1711 bp	1.0	83.5
psbA-matK_p4	CTACAGGAGGAGCTGCAATG	GTTGGCTCCATTGCTATCGA	55.0	50.0	58.0	58.0	1434-1444 bp	0.8	73.7
psbA-matK_p5	CTACAGGAGGAGCTGCAATG	TCGTTGGCTCCATTGCTATC	55.0	50.0	58.0	58.0	1436-1446 bp	0.8	73.7