Hotspot

psaC-ndhE

SSC · 115835-116519 · 685 bp

Back to Trichoglottis

Pi 0.0241

MarkerSeek score 60.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 685
variable_sites: 45
pi: 0.0241
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTCGAACACATTGGGTAC	TCTCAATCTTTGTTATAGCCATTGC	50.0	36.0	57.9	58.0	780-841 bp	1.0	48.5
psaC-ndhE_p2	GCTCGAACACATTGGGTACA	TCTCAATCTTTGTTATAGCCATTGC	50.0	36.0	58.2	58.0	779-840 bp	1.0	48.5
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	CTCAATCTTTGTTATAGCCATTGC	50.0	37.5	57.9	56.7	779-840 bp	1.0	48.5
psaC-ndhE_p4	AGCTCGAACACATTGGGTAC	TCAATCTTTGTTATAGCCATTGC	50.0	34.8	57.9	55.6	778-839 bp	1.0	48.5
psaC-ndhE_p5	TTTCCAATACATCCGTGGGG	TCTCAATCTTTGTTATAGCCATTGC	50.0	36.0	57.6	58.0	802-863 bp	1.0	48.4