Hotspot

rpl32-trnL-UAG

SSC · 112272-112873 · 602 bp

Back to Trichoglottis

Pi 0.0188

MarkerSeek score 64.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 602
variable_sites: 33
pi: 0.0188
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	691-702 bp	1.0	86.5
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	684-695 bp	1.0	86.0
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	690-701 bp	1.0	85.9
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	689-700 bp	1.0	85.9
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	685-696 bp	1.0	85.3