Hotspot

trnN-GUU-rpl32

SSC · 111119-112097 · 979 bp

Back to Trichoglottis

Pi 0.0307

MarkerSeek score 70.50

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 979
variable_sites: 82
pi: 0.0307
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AGTCAGTTAACAGCCAACCG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.1	57.3	1005-1114 bp	1.0	59.1
trnN-GUU-rpl32_p2	ACGACCAGTCAGTTAACAGC	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	1011-1120 bp	1.0	58.8
trnN-GUU-rpl32_p3	AGGATTCGAACCTACGACCA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.2	57.3	1024-1133 bp	1.0	58.7
trnN-GUU-rpl32_p4	AGTCAGTTAACAGCCAACCG	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	58.1	56.2	1012-1121 bp	1.0	58.5
trnN-GUU-rpl32_p5	GGATTCGAACCTACGACCAG	GCTTTTACTGCCTCTAAAGATCC	55.0	43.5	57.8	57.3	1023-1132 bp	1.0	58.3