Hotspot

psbB-psbT

LSC · 75334-75700 · 367 bp

Back to Trichoglottis

Pi 0.0149

MarkerSeek score 51.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 15
pi: 0.0149
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGTATAAACCAATGCTTCCA	45.0	35.0	57.7	52.4	484-509 bp	1.0	45.5
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	ATGTATAAACCAATGCTTCCA	45.0	33.3	57.7	53.1	485-510 bp	1.0	45.5
psbB-psbT_p3	TCAGAGATGTTTTTGCCGGT	TGTATAAACCAATGCTTCCAT	45.0	33.3	57.7	53.1	484-509 bp	1.0	45.5
psbB-psbT_p4	TCAGAGATGTTTTTGCCGGT	AGAAATGTATAAACCAATGCTTCCA	45.0	32.0	57.7	57.3	489-514 bp	1.0	45.4
psbB-psbT_p5	GCCAGAACCTTGTTCAGAGA	TGTATAAACCAATGCTTCCA	50.0	35.0	57.4	52.4	497-522 bp	1.0	45.3