Hotspot

clpP-psbB

LSC · 73005-73806 · 802 bp

Back to Trichoglottis

Pi 0.0244

MarkerSeek score 71.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 53
pi: 0.0244
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	799-947 bp	1.0	86.5
clpP-psbB_p2	TCCTCTCCAGGATTCCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	721-869 bp	1.0	85.3
clpP-psbB_p3	TCCTCTCCAGGATTCCGAAA	ACACGATACCAAGGCAAACC	50.0	50.0	57.8	58.5	724-872 bp	1.0	83.4
clpP-psbB_p4	TCCTCTCCAGGATTCCGAAA	TGAACACGATACCAAGGCAA	50.0	45.0	57.8	57.4	727-875 bp	1.0	82.7
clpP-psbB_p5	ACTGCATCTTCCTCTCCAGG	CAACCAGAAACCAGAGCTGT	55.0	50.0	58.8	58.0	808-956 bp	1.0	82.1