Hotspot

rpoB-trnC-GCA

LSC · 27140-28782 · 1643 bp

Back to Trichoglottis

Pi 0.00869

MarkerSeek score 54.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1643
variable_sites: 41
pi: 0.00869
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACATTCCCTCATTTCCA	CCGGGGATAAAGGATTTGCA	47.6	50.0	57.9	57.9	1572-1738 bp	1.0	76.7
rpoB-trnC-GCA_p2	TGTGGACATTCCCTCATTTCC	CCGGGGATAAAGGATTTGCA	47.6	50.0	57.9	57.9	1573-1739 bp	1.0	76.7
rpoB-trnC-GCA_p3	GTGGACATTCCCTCATTTCCA	ACCGGGGATAAAGGATTTGC	47.6	50.0	57.9	57.9	1573-1739 bp	1.0	76.7
rpoB-trnC-GCA_p4	TGTGGACATTCCCTCATTTCC	ACCGGGGATAAAGGATTTGC	47.6	50.0	57.9	57.9	1574-1740 bp	1.0	76.7
rpoB-trnC-GCA_p5	GTGGACATTCCCTCATTTCCA	TCGAACCGGGGATAAAGGAT	47.6	50.0	57.9	58.2	1577-1743 bp	1.0	75.6