Hotspot

atpH-atpI

LSC · 14173-14795 · 623 bp

Back to Trichoglottis

Pi 0.00818

MarkerSeek score 54.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 14
pi: 0.00818
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.1	57.9	792-827 bp	1.0	79.9
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	741-776 bp	1.0	79.9
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	720-755 bp	1.0	79.4
atpH-atpI_p4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	740-775 bp	1.0	79.3
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	719-754 bp	1.0	78.8