Hotspot

trnR-UCU-atpA

LSC · 10485-10689 · 205 bp

Back to Trichoglottis

Pi 0.0216

MarkerSeek score 56.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 13
pi: 0.0216
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CCGAAGAAGCTGAAACCCTT	47.6	50.0	57.3	58.1	275-304 bp	1.0	73.2
trnR-UCU-atpA_p2	TGGATAGGACAGAGGTCTTCT	CCGAAGAAGCTGAAACCCTT	47.6	50.0	56.9	58.1	310-339 bp	1.0	69.9
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	CCGAAGAAGCTGAAACCCTT	42.9	50.0	56.6	58.1	274-303 bp	1.0	67.6
trnR-UCU-atpA_p4	ATGGATAGGACAGAGGTCTTCT	CCGAAGAAGCTGAAACCCTT	45.5	50.0	57.4	58.1	311-340 bp	1.0	65.9
trnR-UCU-atpA_p5	AGGACAGAGGTCTTCTAAACC	CCGAAGAAGCTGAAACCCTT	47.6	50.0	56.4	58.1	305-334 bp	1.0	65.4