Hotspot

psbC

LSC · 36069-37490 · 1422 bp

Back to Trichocladus

Pi 0.000703

MarkerSeek score 50.50

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTGCAGCAGAAGATCCTGAA	ACCTGATCCGGATCCGAATA	50.0	50.0	57.9	57.7	1588 bp	1.0	76.8
psbC_p2	AAATCCGTGCAGCAGAAGAT	ACCTGATCCGGATCCGAATA	45.0	50.0	57.9	57.7	1594 bp	1.0	76.7
psbC_p3	TTCGTTTCCCAGGAAATCCG	ACCTGATCCGGATCCGAATA	50.0	50.0	58.2	57.7	1607 bp	1.0	76.1
psbC_p4	GTGCAGCAGAAGATCCTGAA	TATGACCTGATCCGGATCCG	50.0	55.0	57.9	58.5	1592 bp	1.0	75.6
psbC_p5	AAATCCGTGCAGCAGAAGAT	TATGACCTGATCCGGATCCG	45.0	55.0	57.9	58.5	1598 bp	1.0	75.5