Hotspot

ccsA

SSC · 118293-119270 · 978 bp

Back to Trichocladus

Pi 0.00102

MarkerSeek score 50.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATGGGATCTTCTAAA	AATGGTTCTCGCCGGATTAC	50.0	50.0	58.0	58.1	1163 bp	1.0	79.4
ccsA_p2	GCGGCATGGGATCTTCTAAA	CGTCAGGCCCTTTCTTCAAT	50.0	50.0	58.0	58.2	1103 bp	1.0	78.4
ccsA_p3	GCGGCATGGGATCTTCTAAA	GATTCAAATGGTTCTCGCCG	50.0	50.0	58.0	57.5	1169 bp	1.0	76.1
ccsA_p4	GCGGCATGGGATCTTCTAAA	ATGTTTGTCTTCGTCAGGCC	50.0	50.0	58.0	58.5	1114 bp	1.0	76.0
ccsA_p5	GCGGCATGGGATCTTCTAAA	CTTCGTCAGGCCCTTTCTTC	50.0	55.0	58.0	58.6	1106 bp	1.0	75.4