Hotspot

ccsA-ndhD

SSC · 119271-119515 · 245 bp

Back to Trichocladus

Pi 0.0449
MarkerSeek score 67.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
245
variable_sites
11
pi
0.0449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GCAAATTCCGCAATTGTAGCT TCAGTTGACAAGGTCGAAACT 42.9 42.9 58.1 57.5 413-416 bp 1.0 60.9
ccsA-ndhD_p2 GCAAATTCCGCAATTGTAGC TCAGTTGACAAGGTCGAAACT 45.0 42.9 56.6 57.5 413-416 bp 1.0 59.0
ccsA-ndhD_p3 GCAATTGTAGCTTCTATGGGC TCAGTTGACAAGGTCGAAACT 47.6 42.9 57.4 57.5 404-407 bp 1.0 56.8
ccsA-ndhD_p4 GCAAATTCCGCAATTGTAGCT TCAGTTGACAAGGTCGAAAC 42.9 45.0 58.1 55.9 413-416 bp 1.0 56.7
ccsA-ndhD_p5 GCAAATTCCGCAATTGTAGCT CAGTTGACAAGGTCGAAACT 42.9 45.0 58.1 55.9 412-415 bp 1.0 56.6