Hotspot

psbE-petL

LSC · 67695-68590 · 896 bp

Back to Trichocladus

Pi 0.212

MarkerSeek score 81.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 185
pi: 0.212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1761-1766 bp	1.0	91.8
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1760-1765 bp	1.0	91.8
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	TGATCACCACGCCTGTATTG	45.0	50.0	58.0	58.0	1545-1550 bp	1.0	91.8
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	TGATCACCACGCCTGTATTG	45.0	50.0	58.0	58.0	1544-1549 bp	1.0	91.8
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	GATCACCACGCCTGTATTGT	45.0	50.0	58.0	58.0	1543-1548 bp	1.0	91.8