Hotspot

rbcL-accD

LSC · 59153-59769 · 617 bp

Back to Trichocladus

Pi 0.00324

MarkerSeek score 60.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 2
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCACCGT	55.0	45.0	58.0	57.8	786-792 bp	1.0	78.5
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TAGAGCTTTCTTGCCCCCTA	55.0	50.0	58.0	57.7	759-765 bp	1.0	77.8
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	CATAGAGCTTTCTTGCCCCC	55.0	55.0	58.0	58.3	761-767 bp	1.0	77.4
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	CCATAGAGCTTTCTTGCCCC	55.0	55.0	58.0	58.3	762-768 bp	1.0	77.4
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	AGAGCTTTCTTGCCCCCTAT	55.0	50.0	58.0	58.4	758-764 bp	1.0	76.7