Hotspot

atpB-rbcL

LSC · 56974-57724 · 751 bp

Back to Trichocladus

Pi 0.00543
MarkerSeek score 58.60
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
751
variable_sites
4
pi
0.00543
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.0 58.0 886-897 bp 1.0 73.2
atpB-rbcL_p2 GGGGGAAAAGCTACATCCAG CACCAGCTTTGAATCCAACAC 55.0 47.6 57.9 58.0 899-910 bp 1.0 73.1
atpB-rbcL_p3 CATCCAGTACCGGACCAATG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.0 58.1 885-896 bp 1.0 72.9
atpB-rbcL_p4 GGGGGAAAAGCTACATCCAG ACCAGCTTTGAATCCAACACT 55.0 42.9 57.9 58.1 898-909 bp 1.0 72.8
atpB-rbcL_p5 TCCAGTACCGGACCAATGAT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 884-895 bp 1.0 72.6