Hotspot

psbM-trnD-GUC

LSC · 30812-31969 · 1158 bp

Back to Trichocladus

Pi 0.00175

MarkerSeek score 61.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCCGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.3	58.5	1253-1269 bp	1.0	75.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCCGTAGC	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.3	57.0	1254-1270 bp	1.0	71.0
psbM-trnD-GUC_p3	AGAATGAACAGTGCCGTAGC	TCAATTGGTCAGAGCACCG	50.0	52.6	58.3	57.8	1252-1268 bp	1.0	69.1
psbM-trnD-GUC_p4	TAGAATGAACAGTGCCGTAGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.1	58.5	1254-1270 bp	1.0	68.4
psbM-trnD-GUC_p5	ACTAGAATGAACAGTGCCGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.9	58.5	1256-1272 bp	1.0	68.3