Hotspot

rpl32-trnL-UAG

SSC · 115462-116951 · 1490 bp

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Pi 0.0165
MarkerSeek score 58.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1490
variable_sites
64
pi
0.0165
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACAGCATTGAAAGCTTT AGCGTGTCTACCGATTTCAC 40.0 50.0 56.1 58.0 1603-1652 bp 1.0 69.6
rpl32-trnL-UAG_p2 TTGGACAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 40.0 50.0 56.1 58.0 1604-1653 bp 1.0 69.6
rpl32-trnL-UAG_p3 TGGACAGCATTGAAAGCTTT CAGCGTGTCTACCGATTTCA 40.0 50.0 56.1 58.0 1604-1653 bp 1.0 69.6
rpl32-trnL-UAG_p4 TTGGACAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 40.0 50.0 56.1 58.0 1605-1654 bp 1.0 69.6
rpl32-trnL-UAG_p5 TGGACAGCATTGAAAGCTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 56.1 57.9 1613-1662 bp 1.0 68.6