Hotspot

ndhF-rpl32

SSC · 114518-115302 · 785 bp

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Pi 0.0215
MarkerSeek score 66.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
785
variable_sites
45
pi
0.0215
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCCCAACGCGATTAGTTACA TCCTCTTGATCGGGTCAAAAC 50.0 47.6 58.0 57.9 1692-1711 bp 0.833333 68.7
ndhF-rpl32_p2 AACAGGTCGTGTGAACCAAA TCCTCTTGATCGGGTCAAAAC 45.0 47.6 57.9 57.9 1646-1665 bp 0.833333 68.0
ndhF-rpl32_p3 ACAGGTCGTGTGAACCAAAA TCCTCTTGATCGGGTCAAAAC 45.0 47.6 57.9 57.9 1645-1664 bp 0.833333 68.0
ndhF-rpl32_p4 CCCCAACGCGATTAGTTACA CCTCTTGATCGGGTCAAAACA 50.0 47.6 58.0 58.2 1691-1710 bp 0.833333 67.4
ndhF-rpl32_p5 GGTCGTGTGAACCAAAAACC TCCTCTTGATCGGGTCAAAAC 50.0 47.6 57.8 57.9 1642-1661 bp 0.833333 67.3