Hotspot

accD-psaI

LSC · 60741-61634 · 894 bp

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Pi 0.0173
MarkerSeek score 57.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
894
variable_sites
37
pi
0.0173
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AACGCCTACATTGCATTTGC CCATTGCAATTGCCGGAAAT 45.0 45.0 58.0 58.0 1142-1233 bp 1.0 86.7
accD-psaI_p2 GGCGAGTTATTTCAGCTCCA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 995-1086 bp 1.0 86.6
accD-psaI_p3 TCTGGGCGAGTTATTTCAGC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 999-1090 bp 1.0 86.6
accD-psaI_p4 TGGGCGAGTTATTTCAGCTC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 997-1088 bp 1.0 86.6
accD-psaI_p5 GGTGACAGCCAGTTTTGGTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 1191-1282 bp 1.0 86.5