Hotspot

rbcL-accD

LSC · 58413-59234 · 822 bp

Back to Trema

Pi 0.00892
MarkerSeek score 57.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
822
variable_sites
18
pi
0.00892
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGCTTGTGAAGTGTGGAAGG TAAATCGAATCGCCGCTTGT 50.0 45.0 58.0 58.4 857-904 bp 1.0 82.6
rbcL-accD_p2 GCTTGTGAAGTGTGGAAGGA TAAATCGAATCGCCGCTTGT 50.0 45.0 58.0 58.4 856-903 bp 1.0 82.5
rbcL-accD_p3 GAGTCCTGAACTAGCTGCAG TAAATCGAATCGCCGCTTGT 55.0 45.0 57.7 58.4 875-922 bp 1.0 80.4
rbcL-accD_p4 AGCTTGTGAAGTGTGGAAGG TTAAATCGAATCGCCGCTTG 50.0 45.0 58.0 57.3 858-905 bp 1.0 79.6
rbcL-accD_p5 GCTTGTGAAGTGTGGAAGGA TTAAATCGAATCGCCGCTTG 50.0 45.0 58.0 57.3 857-904 bp 1.0 79.5