Hotspot

rps4-trnT-UGU

LSC · 47885-48406 · 522 bp

Back to Trema

Pi 0.0135
MarkerSeek score 65.60
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
522
variable_sites
15
pi
0.0135
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CGGTAAAGCTCCCAGATGAC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 637-661 bp 1.0 83.2
rps4-trnT-UGU_p2 GGTAAAGCTCCCAGATGACG CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 636-660 bp 1.0 83.2
rps4-trnT-UGU_p3 CGGTAAAGCTCCCAGATGAC GATGGTCATCGGTTCGACTC 55.0 55.0 58.1 58.2 606-630 bp 1.0 83.2
rps4-trnT-UGU_p4 GGTAAAGCTCCCAGATGACG GATGGTCATCGGTTCGACTC 55.0 55.0 58.1 58.2 605-629 bp 1.0 83.2
rps4-trnT-UGU_p5 GAAACGAGGCCCTCTGTAAC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.3 57.9 607-631 bp 1.0 81.7