Hotspot

psaA-ycf3

LSC · 43306-44139 · 834 bp

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Pi 0.0126
MarkerSeek score 55.10
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
834
variable_sites
22
pi
0.0126
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TTTAACTTCTGGTTCCGGCG TTCGATCAAGCTGCCGAATA 50.0 45.0 58.5 57.4 862-1025 bp 1.0 77.2
psaA-ycf3_p2 TTTAACTTCTGGTTCCGGCG GGAGGCTTGGTTCGATCAAG 50.0 55.0 58.5 58.6 872-1035 bp 1.0 76.9
psaA-ycf3_p3 TTTAACTTCTGGTTCCGGCG TCAAGCTGCCGAATATTGGA 50.0 45.0 58.5 57.3 857-1020 bp 1.0 76.3
psaA-ycf3_p4 TTTAACTTCTGGTTCCGGCG GCTCTATCGCTTACTCCTGG 50.0 55.0 58.5 57.3 832-995 bp 1.0 76.3
psaA-ycf3_p5 TTTAACTTCTGGTTCCGGCG TCGATCAAGCTGCCGAATAT 50.0 45.0 58.5 57.2 861-1024 bp 1.0 75.4