Hotspot

trnG-GCC-trnfM-CAU

LSC · 37936-38139 · 204 bp

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Pi 0.0203
MarkerSeek score 59.50
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
204
variable_sites
11
pi
0.0203
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 223-282 bp 1.0 85.6
trnG-GCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG TGAGGTCACGGGTTCAAATC 50.0 50.0 57.8 57.8 221-280 bp 1.0 84.5
trnG-GCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.8 57.8 244-303 bp 1.0 83.9
trnG-GCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 243-302 bp 1.0 83.9
trnG-GCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG GAGGTCACGGGTTCAAATCC 50.0 55.0 57.8 58.5 220-279 bp 1.0 81.6