Hotspot

trnT-GGU-psbD

LSC · 32615-34235 · 1621 bp

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Pi 0.0122
MarkerSeek score 58.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1621
variable_sites
51
pi
0.0122
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 CCGACCGCCATCATACTATG CCAGACTGACCTAGCGGATA 55.0 55.0 58.0 58.0 2571-2794 bp 0.833333 78.9
trnT-GGU-psbD_p2 CCGACCGCCATCATACTATG AAAGGTTGTACCCGTGAACC 55.0 50.0 58.0 58.0 2236-2459 bp 0.833333 78.9
trnT-GGU-psbD_p3 CCGACCGCCATCATACTATG AAACTAGGCGCAAAGAACCA 55.0 45.0 58.0 58.0 2592-2815 bp 0.833333 78.8
trnT-GGU-psbD_p4 CCGACCGCCATCATACTATG AACTAGGCGCAAAGAACCAA 55.0 45.0 58.0 58.0 2591-2814 bp 0.833333 78.8
trnT-GGU-psbD_p5 CCGACCGCCATCATACTATG ACCAAAACTAGGCGCAAAGA 55.0 45.0 58.0 58.0 2596-2819 bp 0.833333 78.8