Hotspot

petN-psbM

LSC · 29593-30846 · 1254 bp

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Pi 0.00989
MarkerSeek score 57.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1254
variable_sites
30
pi
0.00989
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1318-1439 bp 1.0 73.2
petN-psbM_p2 ACGTTTTCCCTTTCACTCGT TGCTACTGCACTGTTCATTCT 45.0 42.9 57.7 57.6 1280-1401 bp 1.0 71.9
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1257-1378 bp 1.0 71.7
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1318-1439 bp 1.0 69.1
petN-psbM_p5 ACGTTTTCCCTTTCACTCGT TGCTACTGCACTGTTCATTC 45.0 45.0 57.7 56.1 1280-1401 bp 1.0 67.7