Hotspot

rps2-rpoC2

LSC · 16934-17227 · 294 bp

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Pi 0.0259
MarkerSeek score 62.10
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
294
variable_sites
17
pi
0.0259
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCTGCTTCCATCATCTCTTCC TTTGAGTGGGAAATGGGAGA 52.4 45.0 58.2 56.3 392-397 bp 1.0 61.7
rps2-rpoC2_p2 ACTCCTGCTTCCATCATCTCT TTTGAGTGGGAAATGGGAGA 47.6 45.0 58.2 56.3 395-400 bp 1.0 61.0
rps2-rpoC2_p3 TGACCAAAATGAACTCCTGCT TTTGAGTGGGAAATGGGAGA 42.9 45.0 57.8 56.3 407-412 bp 1.0 60.9
rps2-rpoC2_p4 TGAACTCCTGCTTCCATCATC TTTGAGTGGGAAATGGGAGA 47.6 45.0 57.7 56.3 398-403 bp 1.0 60.7
rps2-rpoC2_p5 ACTCCTGCTTCCATCATCTC TTTGAGTGGGAAATGGGAGA 50.0 45.0 56.7 56.3 395-400 bp 1.0 60.5