Hotspot

atpF-atpH

LSC · 13443-13835 · 393 bp

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Pi 0.0224
MarkerSeek score 62.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
393
variable_sites
21
pi
0.0224
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CAAGGAAAGGAAAGGATCGGT TGGTTGTGGCATTAGCACTT 47.6 45.0 57.9 58.0 456-472 bp 1.0 77.4
atpF-atpH_p2 CAAGGAAAGGAAAGGATCGGT TGGACTGGTTGTGGCATTAG 47.6 50.0 57.9 57.8 461-477 bp 1.0 75.8
atpF-atpH_p3 CAAGGAAAGGAAAGGATCGGT CTGGTTGTGGCATTAGCACT 47.6 50.0 57.9 58.5 457-473 bp 1.0 73.7
atpF-atpH_p4 CAAGGAAAGGAAAGGATCGGT GACTGGTTGTGGCATTAGCA 47.6 50.0 57.9 58.5 459-475 bp 1.0 73.7
atpF-atpH_p5 AGGAAAGGAAAGGATCGGTT TGGTTGTGGCATTAGCACTT 45.0 45.0 56.4 58.0 454-470 bp 1.0 73.4