Hotspot

trnS-GCU-trnG-UCC

LSC · 8524-9208 · 685 bp

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Pi 0.0192
MarkerSeek score 63.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
685
variable_sites
28
pi
0.0192
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TATTCACCAAACCGAGGGAC 50.0 50.0 58.0 57.2 735-801 bp 1.0 80.6
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TATTCACCAAACCGAGGGAC 50.0 50.0 58.0 57.2 734-800 bp 1.0 80.6
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TATTCACCAAACCGAGGGAC 50.0 50.0 58.2 57.2 789-855 bp 1.0 79.3
trnS-GCU-trnG-UCC_p4 ACGAAAACGGAAAGAGAGGG TATTCACCAAACCGAGGGAC 50.0 50.0 57.8 57.2 795-861 bp 1.0 79.1
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC TATTCACCAAACCGAGGGAC 50.0 50.0 58.4 57.2 737-803 bp 1.0 77.8