Hotspot

trnH-GUG-psbA

LSC · 78-372 · 295 bp

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Pi 0.0169
MarkerSeek score 56.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
295
variable_sites
13
pi
0.0169
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 TCACAATCCACTGCCTTGAG TCCCTCTAGACTTAGCTGCT 50.0 50.0 57.8 56.9 346-412 bp 1.0 76.1
trnH-GUG-psbA_p2 TCACAATCCACTGCCTTGAG CCCTCTAGACTTAGCTGCTG 50.0 55.0 57.8 56.8 345-411 bp 1.0 75.8
trnH-GUG-psbA_p3 TCACAATCCACTGCCTTGAG ACCGTGCTAATCTTGGTATGG 50.0 47.6 57.8 57.8 397-463 bp 1.0 75.3
trnH-GUG-psbA_p4 TCACAATCCACTGCCTTGAG AGCTGCTGTAGAAGTTCTGTC 50.0 47.6 57.8 57.7 333-399 bp 1.0 74.8
trnH-GUG-psbA_p5 TCACAATCCACTGCCTTGAG GCTGCTGTAGAAGTTCTGTCT 50.0 47.6 57.8 57.7 332-398 bp 1.0 74.8